PIAS3 and systemic lupus erythematosus: We aimed to analyze the association between miR-155 and miR-21 and the expression of <i>SOCS1</i>, <i>PTEN</i>, <i>PIAS3</i>, and <i>IL21</i> in PBMC from SLE patients.<h4>Materials and methods</h4>PBMC isolation was performed by density gradient centrifugation using Histopaque-1077, culture overnight, and seeded at a concentration of 1x10<sup>6</sup> cells/mL in 24-well flat-bottom cell culture plates for subsequent stimulation with 0.5 μg/mL ionomycin and 2.5 μg/mL PMA.