In cytotoxicity assays, 8H8_SDIE demonstrated potent NK cell-mediated lysis of CRC cells.<h4>Conclusion</h4>The Fc-optimized anti-CD276 antibody 8H8_SDIE effectively enhanced NK cell reactivity against CD276-positive CRC cells and induced tumor cell lysis in vitro. This evidence concerns the gene CD276 and colorectal carcinoma.