CD4 and immunodeficiency disease: Findings were validated by flow cytometry in an expanded cohort (15 patients vs. 10 controls).<h4>Results</h4>Single-cell RNA sequencing of PBMCs from patients with AIGAs immunodeficiency syndrome identified a comprehensive immune subset profile, including effector memory CD4<sup>+</sup> T cells, naive CD4<sup>+</sup> T cells, regulatory T cells, GNLY<sup>+</sup> CD8<sup>+</sup> Tem, GZMK<sup>+</sup> CD8<sup>+</sup> Tem, naive CD8<sup>+</sup> T cells, naive B cells, memory B cells, plasma cells, ISG<sup>+</sup> atypical B cells, monocytes, and NKT cells.