The strict conservation of the Asn<sub>150</sub> motif across BTV serotypes underscores its functional importance and highlights NS3/NS3A glycosylation as a potential target for broad-spectrum antivirals or vaccine strategies.IMPORTANCEBluetongue virus (BTV) is an economically significant arbovirus that causes hemorrhagic disease in ruminants and restricts global livestock trade. The gene discussed is KRAS; the disease is hemorrhagic disease.