qRT-PCR was used to analyze the expression level of COL6A3 along with apoptosis markers in HCT116 cell lines (human colorectal cancer) by treating Embelin in a dose-dependent manner.<h4>Results</h4>Trained model predicted COL6A3 as a prominent hub gene across all three ML algorithms with high cross validation accuracy (AUC values: > ~0.90), showing the accuracy of predictions and feature selections of the trained model. This evidence concerns the gene COL6A3 and colorectal cancer.