The non-selective ROCK inhibitor fasudil, used for cerebral vasospasm, limits infarct size after myocardial I/R, but the roles of individual ROCK isoforms in limiting infarction and MVO remain unclear.<h4>Aims</h4>To determine the contribution of ROCK2 to myocardial and microvascular obstruction and to assess the vasodilatory potential of ROCK2 inhibition.<h4>Methods</h4>ROCK1/2 expression was analysed in rat hearts by RNAscope. Here, ROCK2 is linked to infarction.