Elevated mRNA levels of the EndMT biomarkers, <i>VIM</i>, <i>SNAI2</i>, and <i>TWIST1</i>, observed in older TM cells were decreased when β3 integrin was knockdown.<h4>Conclusion</h4>These studies suggest that crosstalk between α5β1 and αvβ3 integrin signaling controls expression of αSMA mRNA and protein levels and that β3 integrins may play a role in the development of the fibrogenic phenotype in TM cells and associated with POAG. This evidence concerns the gene ACTA1 and open-angle glaucoma.