Notably, key endothelial structural and regulatory proteins, including AHNAK (160 sites), MYH9 (56 sites), and FLNA (34 sites), exhibited extensive lactylation.<h4>Discussion</h4>This study provides the first comprehensive lactylome profile of Pg-infected HUVECs, identifying lysine lactylation as a novel mechanism linking periodontal infection to endothelial dysfunction. The gene discussed is JUP; the disease is endothelial dysfunction.