<h4>Background</h4>This study aimed to develop and validate a deep learning model based on preoperative MRI to non-invasively predict Telomerase Reverse Transcriptase (TERT) promoter mutation status in glioma patients.<h4>Methods</h4>A retrospective cohort of 100 patients with histologically confirmed high-grade glioma was included. The gene discussed is TERT; the disease is glioma.