To evaluate the detection efficacy of the SDC2/SFRP2/TFPI2 methylation assay in comparison to conventional methods, fecal occult blood test (FOBT) and serum carcinoembryonic antigen (CEA) levels were measured in 110 CRC patients and 100 healthy individuals at Tianjin Medical University General Hospital.<h4>Results</h4>The stool DNA test demonstrated high sensitivity for CRC (94.13%, 95% CI: 91.42-96.03) and AA (59.76%, 95% CI: 48.94-69.70), with 94.74% specificity (95% CI: 90.30-97.21) in the normal controls. This evidence concerns the gene TFPI2 and colorectal carcinoma.