The integration of all three detection methods achieved the highest detection efficacy (AUC = 0.9725).<h4>Conclusion</h4>The combined methylation assay of SDC2, SFRP2, and TFPI2 outperformed FOBT and CEA in detecting CRC and AA, although further optimization is required for AA detection. The gene discussed is TFPI2; the disease is colorectal carcinoma.