This study evaluated the diagnostic performance of a combined SDC2/SFRP2/TFPI2 gene methylation stool DNA test for detecting colorectal cancer (CRC) and advanced adenoma (AA).<h4>Methods</h4>A multicenter, case-control study was conducted involving 409 patients with CRC, 82 patients with AA, and 495 control participants between July 2022 and March 2024. This evidence concerns the gene TFPI2 and colorectal cancer.