Importantly, supportive functional assays showed that NAT10 knockdown, validated by qRT-PCR, was associated with reduced proliferative activity in HCC cells as evidenced by EdU assays, and IHC validation further corroborated its overexpression in clinical tumor specimens compared to adjacent normal tissues.<h4>Conclusions</h4>This study delineates a CNV-associated landscape of RME dysregulation across cancers and establishes a 12-RME diagnostic signature and a 6-gene prognostic model with robust predictive performance. Here, NAT10 is linked to neoplasm.