Genotyping of the TNFAIP3, IRAK1, and TLR4 was performed via mass spectrometry.<h4>Results</h4>Compared to healthy controls, individuals carrying the rs1059703 A allele (odds ratio (OR) = 0.640, 95% confidence interval (CI): 0.458-0.895, p = 0.009) of IRAK1, individuals carrying the rs5029930 C allele (OR = 0.469, 95% CI: 0.298-0.739, p = 0.001), and AC genotype (OR = 0.427, 95% CI: 0.255-0.716, p = 0.001) of TNFAIP3 had a lower RA risk. The gene discussed is TNFAIP3; the disease is rheumatoid arthritis.