<b>Objective</b>: This study evaluated the effects of a 12-week High-Intensity Functional Training (HIFT) program combined with thylakoid supplementation on plasma adipo-myokine levels (Decorin, Myostatin, Follistatin, Activin A, and TGF-β1) in men with obesity. This evidence concerns the gene TGFB1 and obesity due to melanocortin 4 receptor deficiency.