Stromal phenotype, cytokine secretion, tumor growth, invasion, and immune cell infiltration were assessed.<h4>Results</h4>ADSCs cultured in three-dimensional (3D) hydrogels exhibited reduced Caveolin-1 (CAV-1) expression and reprogramming toward a stress-adapted, CAF-like phenotype compared with two-dimensional (2D) cultures. The gene discussed is CAV1; the disease is neoplasm.