<h4>Purpose</h4>The purpose of this research study was to investigate the impact of isoimperatorin on Aspergillus fumigatus (A. fumigatus) keratitis and elucidate its underlying mechanisms via the TLR4/MyD88/IKK/NF-κB signaling pathway.<h4>Materials and methods</h4>The targets of the active ingredient isoimperatorin were identified via network pharmacology. This evidence concerns the gene NFKB1 and keratitis.