This study explored the combined impact of aerobic exercise and anti-PD-L1 antibody administration on immunological and physiological responses in a murine breast cancer model.<h4>Materials and methods</h4>Thirty female BALB/c mice were divided into five experimental groups: Patient control group (n=6), Exercise+induction+ control (n=6), Exercise+induction+exercise (n=6), Exercise+induction+anti-PD-L1 (n=6), and Exercise induction exercise + anti-PD-L1 (n=6). The gene discussed is CD274; the disease is breast cancer.