These findings were validated in a high-fat diet (HFD)-induced MASLD mouse model, which exhibited hallmark features of metabolic dysfunction, including significant body fat accumulation and elevated serum levels of pro-inflammatory cytokines (interleukin-6 [IL-6] and tumor necrosis factor-α[TNF-α]). The gene discussed is TNF; the disease is metabolic dysfunction-associated steatotic liver disease.