Molecular analyses (qPCR/Western blot) assessed PPARγ/Nrf2/NF-κB signaling.<h4>Results</h4>UDCA treatment significantly ameliorated high-fat diet-induced NAFLD, as demonstrated by improved serum ALT/AST levels, attenuated hepatic steatosis, and reduced histopathological damage. The gene discussed is NFKB1; the disease is fatty liver disease.