This study investigated whether Bryostatin, a PKC modulator derived from marine organisms, could enhance CD20 CAR-T cell activity by up-regulating the CD20 antigen on tumor cells and promoting T cell activation, differentiation and function.<h4>Methods</h4>CD20 antigen expression and trogocytosis-mediated membrane transfer were assessed by flow cytometry and immunofluorescence following co‐culture of CD20 CAR‐T cells with Raji or BALL‐1 cells. The gene discussed is PRRT2; the disease is neoplasm.