NFS1 and malaria: This study aimed to characterize temporal trends in both established, Pfcrt and Pfk13, and less well-described potential markers, cysteine desulfurase (Pfnfs) and Pfcoronin, using febrile malaria samples collected across diverse regions of Kenya between 2013 and 2022.<h4>Methods</h4>The temporal trend of these markers of resistance were assessed by screening archived P. falciparum-positive dried blood spots (DBS).