Binary logistic regression models were used to analyze the relationship between fasting glucagon and stroke risk.<h4>Results</h4>Among female T2DM patients, fasting glucagon levels were significantly higher in the T2DM with stroke group compared to the T2DM group (13.38 vs 11.56 pmol/L, P = 0.011). The gene discussed is GCG; the disease is type 2 diabetes mellitus.