<h4>Objective</h4>This study aimed to investigate the association between fasting glucagon levels and the risk of comorbid stroke in hospitalized patients with type 2 diabetes mellitus (T2DM).<h4>Methods</h4>This study included 1,745 T2DM patients hospitalized at Tianjin Medical University General Hospital from September 1, 2022, to September 30, 2025. This evidence concerns the gene GCG and type 2 diabetes mellitus.