Furthermore, <i>in vivo</i> experimental results indicate that interfering with KIF20A can inhibit tumor growth in nude mice.<h4>Conclusion</h4>To our knowledge, this is the first study to reveal the role of KIF20A in tumorigenesis and development from a pan-cancer multi-omics perspective, providing solid theoretical and experimental evidence for KIF20A as a potential anti-cancer therapeutic target. This evidence concerns the gene KIF20A and cancer.