The regulatory influence of PTTG1 on the TGF-β/Smad pathway was analyzed by measuring the expression levels of TGF-β, Smad2, Smad3, and their phosphorylated forms.<h4>Results</h4>PTTG1 expression was significantly upregulated in gastric cancer tissues compared with normal gastric mucosa according to TCGA data and validation in cell lines. This evidence concerns the gene PTTG1 and gastric cancer.