This study aimed to determine how PTTG1 regulates cell proliferation, migration, and epithelial-mesenchymal transition (EMT) through the TGF-β/Smad signaling pathway in gastric cancer.<h4>Methods</h4>PTTG1 expression in gastric cancer was evaluated using The Cancer Genome Atlas (TCGA) datasets and subsequently validated by western blotting in gastric cancer cell lines. The gene discussed is PTTG1; the disease is gastric cancer.