Western blot analysis revealed that AG could enhance ANIT-induced cholestasis by modulating the anti-oxidative system via activation of the PI3K/Akt/Nrf2 pathway and by regulating bile acid metabolism.<h4>Conclusion</h4>This study demonstrated that AG may mitigate ANIT-induced cholestatic liver damage by improving the bile flow rates, decreasing the concentrations of liver function markers and serum enzyme levels, enhancing liver histology, activating Nrf2 via the PI3K/Akt signaling pathway, and controlling bile acid transport. Here, AKT1 is linked to cholestasis.