In vitro experiments were performed on H9C2 cells using an oxygen-glucose deprivation and recovery model, and various assays were used to assess cell viability, apoptosis, and biochemical changes.<h4>Results</h4>Mild hypothermia (35°C) was found to reduce ischemia-reperfusion injury, enhance myocardial function, decrease infarction area, and increase the expression of phosphorylated Cx43 and protein kinase C in myocardial tissue. The gene discussed is GJA1; the disease is infarction.