DN rats exhibited significantly reduced DUSP1 expression and increased ferroptosis-associated alterations, including elevated ACSL4 expression, enhanced lipid peroxidation, and impaired antioxidant capacity, all of which were partially reversed by Fer-1 treatment (p < 0.001).<h4>Conclusions</h4>Our findings indicate that inhibition of ferroptosis attenuates renal injury in DN and is accompanied by altered DUSP1 expression. Here, ACSL4 is linked to liver dysplastic nodule.