DUSP1 expression was analyzed via immunohistochemistry, Western blot, and RT-qPCR.<h4>Results</h4>DN rats exhibited characteristic metabolic disturbances including polydipsia (394.32 ± 9.92 vs. 28.84 ± 2.45 mL/day, p < 0.001), polyuria, and progressive weight loss. Here, DUSP1 is linked to Polydipsia.