To evaluate the activity of CAR-T cells <i>in vivo</i>, tumor-bearing immunodeficient mice were given a single intravenous injection of CAR-T cells, and the tumor burden and CAR-T cell expansion were regularly monitored.<h4>Results</h4>GPC3 was overexpressed in 70% of LUSC tissues, while negatively expressed in all normal tissues. The gene discussed is GPC3; the disease is neoplasm.