CXCR4-targeted [<sup>68</sup>Ga]Ga-Pentixafor was highly specific in delineating CXCR4-high cell line-derived xenografts and patient-derived xenografts (PDXs) via positron emission tomography (PET) imaging, with precise tumor-targeting and persistent retention. This evidence concerns the gene CXCR4 and neoplasm.