The prediction results of TCM-SPred revealed that a natural guaianolide sesquiterpene lactone derivative dehydrocostus lactone (DCL, a main chemical constituent of Aucklandiae Radix) demonstrated significant anti-fibrotic effects in vivo (unilateral ureteral obstruction) and in vitro (TGF-β1-induced epithelial-mesenchymal transition). This evidence concerns the gene TGFB1 and Ureteral obstruction.