Based on these optimized conditions, we collected 1,350 sheep serum samples from various prefecture-level cities in Hubei Province and compared the detection results using both GRA1-iELISA and <i>Tg</i>267410-iELISA.<h4>Results</h4>In this study, we successfully identified TGME49_267410 as a specific diagnostic antigen for <i>Toxoplasma gondii</i> oocyst-derived infections and established a <i>Tg</i>267410-based indirect enzyme-linked immunosorbent assay (<i>Tg</i>267410-iELISA) for antibody detection. Here, TG is linked to infection.