As our knowledge on the immunolocalization of p53 and p21 during T-2 mycotoxin-induced kidney damage in the avian kidney is limited, despite studies on the effects of T-2 mycotoxins on different organs on a molecular level pointing toward the p53-dependent apoptosis pathway, the aim of this study is to investigate the immunolocalization of the two critical apoptosis-regulatory proteins—p53 and p21—in the renal tissue of broiler chickens subjected to T-2 mycotoxin exposure. This evidence concerns the gene CDKN1A and Nephropathy.