The scores of Pittsburgh sleep quality index (PSQI), self-rating depression scale (SDS), self-rating anxiety scale (SAS), 36-iem short form health survey (SF-36) and serum indexes [5-hydroxytryptamine (5-TH), interleukin-6 (IL-6), dopamine (DA)] before and after intervention were collected and compared.<h4>Result</h4>After intervention, PSQI score (P<0.001), SAS score (P<0.001) and SDS score (P<0.001) in the study group were lower than those in the control group, and SF-36 score was higher than that in the control group (P<0.001). This evidence concerns the gene IL6 and major depressive disorder.