This study aimed to evaluate the clinical performance of droplet digital PCR (ddPCR) for detecting <i>Kirsten Rat Sarcoma Viral Proto-Oncogene</i> (<i>KRAS</i>), <i>Neuroblastoma RAS Viral Oncogene Homolog</i> (<i>NRAS</i>), and <i>B-Raf Murine Sarcoma Viral Oncogene Homolog B</i> (<i>BRAF</i>) mutations in circulating tumor DNA (ctDNA) from colorectal cancer patients using liquid biopsy.<h4>Methods</h4>ctDNA was isolated from colorectal cancer (CRC) patients (n = 110) and analyzed for <i>KRAS</i>, <i>BRAF</i>, and <i>NRAS</i> mutations. The gene discussed is KRAS; the disease is colorectal cancer.