Through integrated <i>in vitro</i> and <i>in vivo</i> studies, we systematically assessed the role of SLC40A1 in promoting M1 macrophage polarization and its relationship with tumor suppression, demonstrating that SLC40A1 enhances the response to immunotherapy.<h4>Results</h4>SLC40A1 was found to be more highly expressed in normal ovarian tissues compared with EOC tissues, and its high expression was associated with a favorable prognosis. This evidence concerns the gene SLC40A1 and neoplasm.