MEX3D and glioma: In addition, <i>in vitro</i> experiments, including qRT-PCR, western blotting, proliferation, migration, and invasion assays, were performed in U251 and LN229 glioma cell lines to functionally validate the bioinformatic findings.<h4>Results</h4>All four MEX3 family members were significantly upregulated in glioma tissues compared with normal controls and demonstrated strong diagnostic performance.