The MEX3 family of RNA-binding proteins has been implicated in tumorigenesis and post-transcriptional regulation; however, their comprehensive roles in glioma remain poorly understood.<h4>Methods</h4>Integrated bioinformatic analyses were performed using transcriptomic and clinical data from TCGA, CGGA, and GEO cohorts to evaluate the expression profiles, diagnostic and prognostic value, genetic alterations, molecular interactions, immune infiltration characteristics, and functional pathways associated with MEX3A, MEX3B, MEX3C, and MEX3D. Here, MEX3D is linked to central nervous system cancer.