Moreover, combination of V9302 and CD276 knockout significantly reduced KYSE150 cell-derived xenograft tumor volume by 65.2% (95% CI 58.3-72.1%, <i>P</i> < 0.001) in NOD/SCID mice, without affecting mouse body weight (<i>P</i> > 0.05).<h4>Conclusion</h4>SLC1A5 enhances CD276 stability by suppressing ROS-autophagy signaling, promoting ESCC progression. The gene discussed is SLC1A5; the disease is esophageal squamous cell carcinoma.