This was done in samples from a prospective clinical trial at the Eye Clinic, Umeå University Hospital (Sweden), including 30 controls and 90 patients with glaucoma.<h4>Results</h4>A Western blotting assay was designed, detecting eNAMPT (52 kilodalton [kDa]) and transferrin (77 kDa) as housekeeping protein from 0.2 μL EDTA-plasma. Here, TF is linked to glaucoma.