CD40LG and congenital secretory chloride diarrhea 1: This study assessed HEV infection in CLD patients from Latin America and potential associated factors, including the PROGINS haplotype.<h4>Methods</h4>A total of 971 individuals-784 with CLD and 187 healthy controls (HC)-from six countries (Argentina, Brazil, Chile, Colombia, Ecuador, and Peru) were analyzed for anti-HEV IgG and IgM (ELISA), HEV-RNA (RT-qPCR and nested PCR with Sanger sequencing and phylogenetic analysis), and the PROGINS haplotype (PCR).<h4>Results</h4>The overall anti-HEV IgG seroprevalence was 15.2%: 15.4% in CLD and 14.4% in HC, with no statistical difference.