Study in allergic asthma mice and MHS alveolar macrophages further demonstrate that the damage-associated molecular pattern S100A8/A9 enhances glycolysis and lactate production via TLR4/MyD88/NF-κB signaling, concomitant with increased ACLY phosphorylation and upregulation of M1 signature factors. Here, TLR4 is linked to allergic asthma.