FKBP51 is highly expressed in BT325 cells, which induces cell cycle arrest and reduces tumor cell proliferation by inhibiting the phosphorylation of AKT ser473 and activating the transcription of P21 and P27. At the same time, the overexpression of FKBP51 can increase the sensitivity of BT325 to BCNU through the AKT pathway. On the other hand, FKBP51 can increase the expression of MMP-2 and MMP-9 in BT325 cells and enhance the migration and invasion ability of cells. This evidence concerns the gene AKT1 and neoplasm.