Although MKN45 cells have been widely used for studying TNF-α-induced inflammatory signaling including NF-κB pathway activation (Cho et al., 2023), as a cancer-derived cell line, they may exhibit differences from normal gastric epithelial cells, including altered proliferation rates, constitutive activation of certain oncogenic pathways, and potentially different baseline inflammatory responses. The gene discussed is NFKB1; the disease is cancer.