The results demonstrated that in the GSE39653 dataset, CSPP1 (p < 0.01) and ZNF84 (p < 0.001) were significantly upregulated in MDD samples, while HLA-DPA1 (p < 0.05), CCZ1 (p < 0.05), and LRRC8D (p < 0.01) were significantly downregulated. The gene discussed is LRRC8D; the disease is major depressive disorder.