After the quality control, normalization and cell annotation, a total of 187,290 cells were divided into 14 celltypes, including NK cells, B cells, plasma cells, classical dendritic cells (cDCs), plasma dendritic cells (pDCs), CD4+ T cells, CD8+ T cells, macrophages (Macs), mast cells, endothelial cells (ECs), fibroblasts (FIBs), non-proximal tubule epithelial cells (nonPT-Epi), proximal tubule epithelial cells (PT-Epi), and renal cell carcinoma (RCC) cells (Figure 1A). The gene discussed is CD8A; the disease is hereditary clear cell renal cell carcinoma.