In a study with the construction of an insomnia mouse model via para-Chlorophenylalanine (PCPA) intraperitoneal injection combined with multi-factor stimulation, BDT increased GABA concentration and decreased Glu concentration in the brain of insomniac mice; downregulated the expression levels of IL-1β, IL-6, TNF-α, and NF-κB genes in colon tissue, significantly shortened the sleep latency, and increased the sleep duration of insomniac mice (Wang Y. J. et al., 2024). Here, NFKB1 is linked to insomnia.