Consistently, physalin F inhibited SeV-induced phosphorylation of IRF3 at Ser386 (IRF3S386), STAT1 at Y701 (STAT1Y701), and STAT2 at Y690 (STAT2Y690) at 8 h post-infection in HEK293T cells (Figure 1C, upper panels). This evidence concerns the gene IRF3 and infection.