MAVS and infection: Consistently, comparing to AFG3L2-deficient cells, cells reconstituted with wild-type AFG3L2 but not the protease-inactive AFG3L2E575Q mutant down-regulated VISA level in uninfected and SeV-infected cells, and inhibited SeV-induced phosphorylation of TBK1S172 and IRF3S386 at 6 h post-infection (Figure 5D).