However, the proportion of insulin+/PDX1− cells among all insulin+ cells within the focus and in diffuse CHI observed in the present study (17.66% in focus and 21.54% in diffuse CHI) was significantly higher than the percentage of β-cell apoptosis reported in other studies (<5% apoptotic β-cells in both types of CHI) [15,47]. This evidence concerns the gene INS and congenital isolated hyperinsulinism.