Both inhibitors significantly attenuated WISP1-induced MIF expression, with PP2 reducing MIF levels by ~20% at the mRNA level and ~23% at the protein level, while ISO-1 reduced mRNA and protein levels by ~23% and ~35%, respectively, compared to WISP1 treatment alone (Figure 1C,D), indicating that Src kinases and MIF itself are implicated in WISP1-mediated upregulation of MIF in breast cancer cells. This evidence concerns the gene CCN4 and breast cancer.