To investigate whether WISP1 also regulates MIF expression in breast cancer, non-invasive ER+ MCF7 cells were treated with increasing concentrations of recombinant human WISP1 (125, 250, 500, and 1000 ng/mL) for 24 h, and secreted MIF levels were quantified by ELISA. The gene discussed is MIF; the disease is breast cancer.