Furthermore, our results showed that the components of GLT that regulate core targets are primarily neutral triterpenoids, including Ganoderal A, Ganoderiol F, Ganoderlactone D, Ganodermanondiol, Ganodermanontriol, Lucialdehyde A, Lucialdehyde B, Lucidal, Ganolactone B and 3,7,15-trihydroxy -11-oxo-lanosta-8-en-24-20 lactone, suggesting that neutral triterpenoids may exhibit more potent anti-obesity activity. This evidence concerns the gene NOP53 and obesity due to melanocortin 4 receptor deficiency.