In vivo CRISPR screening of 217 genes in non-metastatic 393P cells singled out TMEM106B as the strongest driver of lung cancer metastasis; its overexpression raises metastatic burden without altering primary tumor growth, whereas knockdown suppresses migration, invasion, and TGF-β-induced invasive structures in 3D cultures both in human and murine lung cancer cell lines [123]. The gene discussed is TGFB1; the disease is lung carcinoma.