While earlier reports have reported the immortalization of peripheral blood lymphocytes from bulk HVS infection, resulting in immortalized CD4+ and CD8+ T-cell recovery [13], these methods were not deemed suitable for our purpose, for two major reasons: first, this approach preferentially selected CD8+ cells from PLWH individuals, likely due to their serological profile [12,13], and more importantly very of the immortalized clones were actually HIV-infected. This evidence concerns the gene CD4 and infection.